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Amprobe GmbH
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Ribobio co
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ACGT Inc
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CloudSeq Biotech Inc
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Organon Teknika Corporation LLC
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Metabion International AG
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Assay Designs Inc
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YSI Inc
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Shanghai GenePharma
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Viagene Inc
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Image Search Results
Journal: Drug Delivery
Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis
doi: 10.1080/10717544.2022.2030428
Figure Lengend Snippet: MiR-141-3p is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Article Snippet: Biotin-labeled
Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, Negative Control, Pull Down Assay, Binding Assay, Sequencing, Luciferase, Reporter Assay, Activity Assay, Over Expression
Journal: Drug Delivery
Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis
doi: 10.1080/10717544.2022.2030428
Figure Lengend Snippet: MiR-141-3p knockdown suppresses the activation of HSCs by PTEN/AKT pathway. (A–G) LX2 cells were transfected with miR-141-3p inhibitor or NC inhibitor. (A) qRT-PCR analysis for the expression level of miR-141-3p in LX2 cells after transfection. (B, C) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (D, E) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (F, G) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.
Article Snippet: Biotin-labeled
Techniques: Activation Assay, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Western Blot
Journal: Drug Delivery
Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis
doi: 10.1080/10717544.2022.2030428
Figure Lengend Snippet: CircDIDO1 suppresses the activation of HSCs by miR-141-3p/PTEN/AKT pathway. (A–F) LX2 cells were co-transfected with circDIDO1 and miR-141-3p mimic. (A, B) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (C, D) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (E, F) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.
Article Snippet: Biotin-labeled
Techniques: Activation Assay, Transfection, CCK-8 Assay, Flow Cytometry, Western Blot
Journal: Drug Delivery
Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis
doi: 10.1080/10717544.2022.2030428
Figure Lengend Snippet: MSC-exosome-mediated transfer of circDIDO1 suppresses the activation of HSCs by PTEN/AKT pathway. (A–H) LX2 cells were co-cultured with PBS, MSC-circDIDO1 Exo or MSC-vector Exo. (A) qRT-PCR analysis of circDIDO1 and miR-141-3p expression level in LX2 cells after incubation. (C, D) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (E, F) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (G, H) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.
Article Snippet: Biotin-labeled
Techniques: Activation Assay, Cell Culture, Plasmid Preparation, Quantitative RT-PCR, Expressing, Incubation, CCK-8 Assay, Flow Cytometry, Western Blot